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. 2023 Oct 7;40(10):msad221. doi: 10.1093/molbev/msad221

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© The Author(s) 2023. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — BtFTSP1 interacts with NtFD1. a) Domain organization of NtFD1. NtFD1 contains a chloroplastic transit peptide (cTP) at the N-terminus and a ferredoxin domain at the C-terminus. b) Yeast 2-hybrid assays showing the interaction between BtFTSP1 and different NtFD1 mutants. c) Co-immunoprecipitation (Co-IP) assays showed the interaction between BtFTSP1 and NtFD1. Total proteins were extracted from Nicotiana benthamiana leaves expressing BtFTSP1-Myc/NtFD1-Flag and BtFTSP1-Myc/GUS-Flag, then precipitated with Flag beads and probed with anti-Flag and anti-Myc antibodies for immunoblot analysis. d, e) Bimolecular fluorescence complementation (BiFC, d), and luciferase complementation (LUC, e) assays confirmed the interaction between BtFTSP1 and NtFD1.