Table 3.

Statistical analysis and characterization of the SO1861-enhanced targeted toxin therapy on prostate cancer cells. Statistical analysis according to the Bliss Independence score revealed the interaction quality based on the difference between the measured and the predicted inhibition rate. Values > 0 indicate synergism, values < 0 indicate antagonism. The cumulative therapy effect of both individual substances (Ea + Eb) minus (EaEb) corresponds to the predicted inhibition rate (Êa+b). For all targeted toxin concentrations tested between 0.0003 and 5 nM in LNCaP cells and 0.039 and 2.5 nM in PC-3 cells, the predicted inhibition rate is equivalent to the inhibition rate of SO1861 alone, as EGF-PE24mutΔREDLK was not cytotoxic in single application. MW ± SEM was calculated from three independent experiments. P-values were calculated by unpaired t-test with Welch's correction.

Abbreviations: E, treatment effect; syn, synergistic; ant, antagonistic; ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.