Table 1. Concentrations and volumes of reagents used in PCR reactions for CanineCV detection.

Reagents	Concentrations	Volume 1X
Ultrapure water	-	15.2 μl
DNTP*	40 mM	2.0 μl
Buffer 10X	10x	2.5 μl
MgCl2**	50 mM	0.8 μl
Primer Rev 533	20 pmol	0.5 μl
Primer For Genomic	20 pmol	0.5 μl
Taq DNA polymerase	5U/ μl	0.5 μl

^*DNTP - Phosphate deoxyribonucleotides

^**Mgcl2 - Magnesium chloride.