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| Parameters | Temperatures | Time |
|---|---|---|
| Initial denaturation | 94°C | 5 minutes |
| Amplification cycles (40x) | ||
| Denaturation | 94°C | 30 seconds |
| Annealing | 55°C | 1 minute |
| Extension | 72°C | 1 minute |
| Final extension | 72°C | 5 minutes |
