Extended Data Table 1 |.
Cryo-EM data collection, refinement and validation statistics
| TnsC (single heptamer) (EMDB-24783) (PDB 7RZY) |
TnsC-DNA (single heptamer) (EMDB-26476) (PDB 7UFI) |
TnsC-DNA (double heptamer) (EMDB-26477) (PDB 7UFM) |
|
|---|---|---|---|
| Data collection and processing | |||
| Magnification | 105,000 | 130,000 | |
| Voltage (kV) | 300 | 300 | |
| Electron exposure (e–/Å2) | 53.55 | 73.94 | |
| Defocus range (μm) | −0.5/−2 | −0.5/−2 | |
| Pixel size (Å) | 0.678 | 0.6485 | |
| Symmetry imposed | C7 | C1 | C1 |
| Initial particle images (no.) | 2.4M | 4.2M | 4.2M |
| Final particle images (no.) | 84,309 | 125,371 | 125,833 |
| Map resolution (Å) | 3.54 | 3.46 | 3.92 |
| FSC threshold | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) | 3-5 | 3.5-6 | 4-8 |
| Refinement | |||
| Initial model used (PDB code) | Manual built | Manual built | Manual built |
| Model resolution (Å) | 3.9 | 4.2 | 5.3 |
| FSC threshold | 0.5 | 0.5 | 0.5 |
| Model resolution range (Å) | 3-5 | 3.5-5 | 4-8 |
| Map sharpening B factor (Å2) | −111 | −85.23 | −112.5 |
| Model composition | |||
| Non-hydrogen atoms | 17,458 | 18,319 | 36,392 |
| Protein residues | 2,111 | 2,177 | 4,354 |
| Ligands | ATP (7 molecules) | ATP (7 molecules) | ATP (14 molecules) |
| DNA nucleotides | - | 21 | 44 |
| B factors (Å2) | |||
| Protein | 84.23 | 74.49 | 129.21 |
| Ligand | 65.70 | 53.53 | 108.9 |
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.014 | 0.014 | 0.0087 |
| Bond angles (°) | 1.73 | 1.81 | 1.82 |
| Validation | |||
| MolProbity score | 1.54 | 2.41 | 3.05 |
| Clashscore | 1.86 | 2.75 | 7.84 |
| Poor rotamers (%) | 0.74 | 1.22 | 1.92 |
| Ramachandran plot | |||
| Favored (%) | 88.40 | 90.38 | 89.00 |
| Allowed (%) | 98.57 | 99.49 | 11.00 |
| Disallowed (%) | 1.43 | 0.51 | 0 |