TABLE 2.
ESR reporter activity during ampicillin and meropenem treatment a
| Reporter gene | ESR | Control | Amp 100 µg/mL | Mpn 6 µg/mL | |
|---|---|---|---|---|---|
| Inducer | Fold-change | Fold-change | Fold-change | ||
| micA | σE | Ethanol | 2.8 | 1.8 | 2.4 |
| spy | Bae | Ethanol | 15.0 | 1.5 | 1.2 |
| pspA | Psp | Isohexane | 18.3 | 1.1 | 1.2 |
| cpxP | Cpx | Zinc sulfate | 5.0 | 1.1 | 1.2 |
| rcsA | Rcs | Poly B | 5.0 | 1.1 | 1.8 |
| pro1p | N.A. | Ethanol | 1.5 | 1.0 | 1.1 |
| Isohexane | 1.3 | ||||
| Zinc sulfate | 1.1 | ||||
| Poly B | 1.1 | ||||
a
Activation of ESR-dependent fluorescent transcriptional reporters by 60-min treatment with positive controls (inducer) and ampicillin (Amp) at 100 µg/mL or meropenem (Mpn) at 6 µg/mL, measured by fluorescence microscopy. The reporter genes allowing monitoring of the induction of each ESR are indicated. The synthetic promoter pro1p (pro1p-mNG) was used as an internal control. Data extracted from Fig. 2; Fig. S6. Poly B, Polymyxin B.