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. 2023 Sep 13;67(10):e00111-23. doi: 10.1128/aac.00111-23

TABLE 1.

Characterization of donor E. coli strain, evolved and ancestral E. coli UB12 clones, and new in vivo transconjugants used in this study

Status Isolate name Experiment
(month/year)		 Box a Animal a Day of evolution Phylogroup MLST type b Serotype FimH allele Plasmid replicon c Antimicrobial resistance genes Mobility d Biofilm formation e (SD)
Ancestral strains M63-DN - - - B1 ST2521 O147:H7 fimH31 IncI (KJ484635); ColRNAI (J01566) bla CTX-M-1 ,sul 2, aadA5, dfrA17  + 0.079 (+/− 0.055)
UB12-RC - - - B1 ST2520 O100:H40 fimH31 IncY (MH42254); Col156 (EU090225) -  + 0.114 (+/− 0.066)
UB12-TC - - - B1 ST2520 O100:H40 fimH31 IncI (KJ484635); ColRNAI (J01566); IncY (MH42254); Col156 (EU090225) bla CTX-M-1 ,sul 2, aadA5, dfrA17  +/− 0.081 (+/− 0.048)
Evolved strains with IncI plasmid UB12-TCive.1 January/2013 F3v 4368 D28 B1 ST2520 O100:H40 fimH31 IncI (KJ484635); ColRNAI (J01566); IncY (MH42254); Col156 (EU090225) bla CTX-M-1 ,sul 2, aadA5, dfrA17  +/− 0.094 (+/− 0.047)
UB12-TCive.2 June/2013 B2d 238 D8  +/− 0.092 (+/− 0.048)
UB12-TCive.3 June/2013 B4v 4631 D31  +/− 0.091 (+/− 0.044)
UB12-TCive.4 October/2012 B1 4219 D7  +/− 0.090 (+/− 0.048)
UB12-TCive.5 October/2012 B1 4219 D20  +/− 0.092 (+/− 0.042)
UB12-TCive.6 December/2015 C3d 462 D18  +/− 0.086 (+/− 0.043)
UB12-TCive.7 December/2015 C4v 5652 D45  +/− 0.091 (+/− 0.053)
Evolved strains with IncI plasmid loss UB12-TCΔpIncI.1 June/2013 B2v 4613 D20 B1 ST2520 O100:H40 fimH31 ColRNAI (J01566); IncY (MH42254); Col156 (EU090225) -  + 0.100 (+/− 0.067)
UB12-TCΔpIncI.2 April/2012 C3v 3716 D7  + 0.104 (+/− 0.054)
       UB12-TCΔpIncI.3 April/2012 C3v 3716 D18  + 0.095 (+/− 0.041)
New in vivo
transconjugants with IncI plasmid		 NI.1 January/2013 B2v 4331 D22 D ST13022 -:H25 fimH123 IncI (KJ484635); IncY (MH42254); IncFII (pRSB107) bla CTX-M-1, sul 2, aadA5, dfrA17  + ND
NI.2 January/2013 F3v 4324 D22 A ST48 O184:H11 fimH41 IncI (KJ484635); IncFIC(FII), IncFIB (AP001918); IncFII (pSFO) (AF401292); ColE10 (X01654); ColRNAI (J01566) bla CTX-M-1, sul 2, aadA5, dfrA17, sul1, tetA, tetB  +/− ND
NI.3 January/2013 F3v 4368 D10 B1 ST13023 -:H7 fimH68 IncI (KJ484635) bla CTX-M-1, sul 2, aadA5, dfrA17  + ND
NI.4 June/2013 B2v 4591 D16 B1 ST2540 OgN9:H2 fimH31 IncI (KJ484635); ColRNAI (J01566) bla CTX-M-1, sul 2, aadA5, dfrA17  + ND
NI.5 June/2013 B2v 4575 D13 G ST657 O149:H25 fimH97 IncI (KJ484635); IncFII(pRSB107)(AJ851089); ColRNAI (J01566); Col(MG828) bla CTX-M-1, sul 2, aadA5, dfrA17  + ND
NI.6 June/2013 B2d 238 D8 C ST410 Onovel14:H9 fimH24 IncI (KJ484635); IncFIC(FII), IncFIB (AP001918), IncFII (pSFO) (AF401292); ColRNAI (J01566) bla CTX-M-1, sul 2, aadA5, dfrA17, tetA  − ND
NI.7 October/2012 B1 4219 D20 G ST117 O24:H4 fimH97 IncI (KJ484635); IncFIC(FII), IncFIB, IncF1A (AP001918), IncFII (pSFO) (AF401292); ColRNAI (J01566); Col(MG828) bla CTX-M-1, sul 2, aadA5, dfrA17  + ND
NI.8 December/2015 C4d 5625 D4 B1 ST767 O8:H9 fimH32 IncI (KJ484635); IncFIC(FII), IncFIB (AP001918); IncFII (pSFO) (AF401292); Col(MG828) bla CTX-M-1, sul 2, aadA5, dfrA17, tetA  + ND
NI.9 December/2015 C4d 5625 D11 B2 ST95 O18:H7 fimH15 IncI (KJ484635); IncFIC(FII), IncFIB (AP001918), IncFII (pSFO) (AF401292); ColRNAI (J01566) bla CTX-M-1, sul 2, aadA5, dfrA17  +/− ND
NI.10 December/2015 C4v 5626 D11 G ST117 -:H19 fimH97 IncI (KJ484635); IncFIB (AP001918), IncFII (pRSB107); Col(MG828); Col8282 (DQ995353); p0111; ColRNAI (J01566) bla CTX-M-1, sul 2, aadA5, dfrA17, tetB  − ND
NI.11 December/2015 C4d 5651 D11 C ST88 O8:H9 fimH39 IncI (KJ484635) bla CTX-M-1, sul 2, aadA5, dfrA17  −/+ ND
a

Detailed in references (9, 11).

b

According to the Warwick multilocus sequence typing (MLST) scheme.

c

PlasmidFinder, IncI plasmid is in bold.

d

+, total spreading within medium; +/−, partial spreading; −, not spreading.

e

Mean of absorbance measure ancestral tranconjugants at 570 nm. SD, standard deviation; ND, not determined.