Fig 1.

Fluo-4 induced Ca²⁺ signals in Brugia muscles. (A) Micrograph of a fluorescing dissected Brugia muscle injected with 5 µM Fluo-4 under blue light. Key structures of the body, nucleus, and muscle arms are indicated with the arrows. The red box indicates the region of interest (ROI) where we take the calcium recording. ROI is determined as the clearest part of the muscle cell with good Fluo-4 fluorescence. (B) Representative control trace of Fluo-4 fluorescence in a muscle being washed with bath solution containing 1 mM CaCl₂ followed by an increase to 10 mM CaCl₂ for 1 minute (gray box). (C) Total amplitudes of Fluo-4 fluorescence in response to constant 1 mM CaCl₂ bath solution perfusion (black bar) and to 10 mM CaCl₂ (gray bar). *** indicates significantly different to 1 mM CaCl₂ bath solution (bath solution vs CaCl₂). P < 0.001, t = 8.817, df = 5, paired t-test. n = 6, individual muscles from six individual Brugia females. All values are represented as means ± standard error of the mean (SEM).