Fig 4.

Inhibition of TRP-2 ablates diethylcarbamazine sensitivity: (A) Representative trace showing the application of 10 µM SKF96365 (light brown bar) for 5 minutes alone followed by the combination of SKF96365 and of 30 µM diethylcarbamazine (light brown box) for 5 minutes. SKF96365 is removed leaving diethylcarbamazine alone for a final 5-min exposure. Ten millimolar CaCl₂ control was applied for 1 minute (gray box) as a positive control. (B) Total Ca²⁺ amplitudes in response to SKF96365 + diethylcarbamazine (SKF + DEC) (light brown bar) and CaCl₂ control (gray bar). * indicates significantly different to SKF96365 + diethylcarbamazine. SKF96365 + diethylcarbamazine (SKF + DEC) vs CaCl₂: P < 0.002, t = 4.480, df = 8, paired t-test. n = 9 for diethylcarbamazine + SKF96365 responses and n = 9 for CaCl₂ responses from nine individual muscles from nine individual Brugia. All values are represented as means ± SEM.