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EC50,off→on over time simulated from the final model based on the C/T in vitro sequential time-kill data. Time is defined as the time since the beginning of the first time-kill. Each colored line shows the empirical EC50 for a different strain. Dashed vertical line shows time = 24 hours which is the time at which samples from the first time-kill are taken to prepare the inoculum of the second time-kill.
