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Gap junction communication between cumulus cells and oocytes assessed by Calcein AM. (A) COCs were incubated with Calcein AM for 10 min or 20 min. After removing the cumulus cells, the green fluorescence of Calcein AM transferred to oocytes through gap junctions was detected. Scale bar = 80 µm. (B) The average fluorescence intensity of Calcein AM in oocytes measured using Image J image analysis (N = 10).
Compared with control, *P < 0.05.
