Figure 2.

Dose–response curves of NVP, 2-nitro-NVP, 12-d₃-NVP, and 3-bromo-NVP against HIV reverse transcriptase. HIV-RT inhibition by NVP (A), 2-nitro-NVP (B), 12-d₃-NVP (C), and 3-bromo-NVP (D) was characterized by the following IC₅₀ value ± standard error (with respective coefficient of determination, R², in parentheses): 0.540 ± 0.055 μM (R² = 0.972), 0.721 ± 0.111 μM (R² = 0.937), 0.910 ± 0.042 μM (R² = 0.989), and 2.78 ± 0.38 μM (R² = 0.977), respectively. Each data point represents the average of triplicate measurements, with error bars representing standard deviations. NVP, nevirapine; HIV-RT, HIV reverse transcriptase.