Figure 4.
Induction effects of NVP and its analogs on CYP2B6 gene expression in primary human hepatocytes. Fold change of CYP2B6 mRNA in pooled primary human hepatocytes dosed for 72 h with 25 and 100 μM NVP, 2-nitro-NVP, 12-d3-NVP, or 3-bromo-NVP. DMSO and rifampicin (10 μM) treatments were used for solvent control and positive control for induction, respectively. Bars represent the means of at least four biological replicates ± standard deviations. Data were analyzed by one-way ANOVA against the DMSO control. Statistical difference is shown by **** corresponding to the p < 0.0001 significance. ns, not significant; NVP, nevirapine; RIF, rifampicin.