Table 1.
TaqMan probes and primers used for RT-qPCR assays
| Gene1 | TaqMan probe assay | Manufacturer |
|---|---|---|
| RPS9 | Bt03272016_m1 | Thermo Fisher |
| HMBS | Bt03234763_m1 | |
| HSP20 | Bt03213719_m1 | |
| HSP27 | Bt03220563_m1 | |
| HSP70 | Bt03292670_g1 | |
| HSP90 | Bt03244099_g1 | |
| MyoD | Bt03244740_m1 | |
| MyoG | Bt03258928_m1 | |
| IGF-1 | Bt03252282_m1 | |
| Myf5 | Bt03223134_m1 | |
| P70 S6K | Bt00923436_m1 | |
| PPARγ | Bt03217547_m1 | |
| C/EBPα | Bt03224529_s1 | |
| FAS | Bt03210481_m1 | |
| SCD | Bt04307476_m1 | |
| MHC Ⅰ | Bt03224257_m1 | |
| Genes | Primer and probe sequence (5’ to 3’) | Manufacturer |
| Pax7 | ||
| Forward | GCCCTCAGTGAGTTCGATTAG | |
| Reverse | GATGCTGTGCTTGGCTTTC | |
| Probe | 6FAM-TTCGTCCTCCTCCTCCTTCTTCCC-MGBNFQ | |
| MHC ⅡA | Thermo Fisher | |
| Forward | GCAATGTGGAAACGATCTCTAAAGC | |
| Reverse | GCTGCTGCTCCTCCTCCTG | |
| Probe | 6FAM-TCTGGAGGACCAAGTGAACGAGCTGA-TAMRA | |
| MHC ⅡX | ||
| Forward | GGCCCACTTCTCCCTCATTC | |
| Reverse | CCGACCACCGTCTCATTCA | |
| Probe | 6FAM-CGGGCACTGTGGACTACAACATTACT-TAMRA | |
1 RPS9: ribosomal protein subunit 9; HMBS: hydroxymethylbilane synthase; HSP: heat shock protein; MyoD: myoblast determination protein 1; MyoG: myogenin; IGF-1: insulin-like growth factor 1; Myf5: myogenic factor 5; P70S6K: ribosomal protein S6 kinase beta 1; PPARγ: peroxisome proliferator activated receptor gamma; C/EBPα: CCAAT/enhancer-binding protein-alpha; FAS: fatty acid synthase; SCD: stearoyl-coenzyme A desaturase; Pax7: paired box gene 7; MHC: myosin heavy chain.