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. 1966 Sep;14(5):742–745. doi: 10.1128/am.14.5.742-745.1966

Effect of Diluent and Relative Humidity on Apparent Viability of Airborne Pasteurella pestis

William D Won 1, Harold Ross 1
PMCID: PMC1058407  PMID: 5970462

Abstract

Airborne Pasteurella pestis (A-1122) at low humidities [20 to 50% relative humidity (RH)] exhibited exponential decay when either 1% peptone or Heart Infusion Broth (HIB) was used as the diluent in the viable assay system. At higher RH values (65 and 87%), however, the 1% peptone diluent adversely affected the viability assay. In contrast, HIB as diluent was remarkably effective in demonstrating a higher number of viable cells in aerosols held at high RH values. Similarly, with HIB as diluent, aerosols were shown to contain viable cells during 90 min of observation; with 1% peptone, viability was not detectable after 20 min in the airborne state.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. MCDADE J. J., HALL L. B. SURVIVAL OF GRAM-NEGATIVE BACTERIA IN THE ENVIRONMENT. I. EFFECT OF RELATIVE HUMIDITY ON SURFACE-EXPOSED ORGANISMS. Am J Hyg. 1964 Sep;80:192–204. doi: 10.1093/oxfordjournals.aje.a120468. [DOI] [PubMed] [Google Scholar]
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