KEY RESOURCES.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Antibodies | ||
|
| ||
| Anti-Fluorogold | Millipore | Cat. # AB153; RRID AB_2314412 |
| Biotinylated goat anti-rabbit | Vector Labs | Cat. # BA1000; RRID AB_2313606 |
| Anti-cFos | Cell Signaling Technologies | Cat. # mAB5348; RRID:AB_10557109 |
| anti-TH antibody | Millipore | Cat. # MAB318; RRID:AB_2201528 |
| anti-Calbindin | Abcam | Cat. # ab156812; RRID:AB_2747594 |
| anti-Substance-P | Neuromics | Cat. # P14103; RRID: AB_2629484 |
| 647 Alexa Fluor anti-mouse | Jackson ImmunoResearch Labs | Cat. # 71560517; RRID:AB_2338075 |
| 594 Alexa Fluor anti-guinea pig | Jackson ImmunoResearch Labs | Cat. # 706585148; RRID:AB_2340474 |
| 488 Alexa Fluor anti-rabbit | Jackson ImmunoResearch Labs | Cat. # 711545152; RRID:AB_2313584 |
| 405 Dylight anti-goat | Jackson ImmunoResearch Labs | Cat. # 705475147; RRID:AB_2340427 |
| anti-mCherry | Takara | Cat. # 632543; RRID:AB_2307319 |
| anti-VGluT2 | Frontier Institute | VGluT2-Go-Af310; RRID: AB_2571620 |
| Anti-VGaT | Frontier Institute | Cat# VGAT-GP, RRID:AB_2571624 |
| anti-TH | Millipore | Cat. # AB152; RRID:AB_390204 |
| DyLight-405-donkey anti-guinea pig | Jackson ImmunoResearch Labs | Cat# 706-475-148, RRID:AB_2340470 |
| Alexa-Fluor-594-donkey anti-mouse | Jackson ImmunoResearch Labs | Cat# 715-585-151, RRID:AB_2340855 |
| Alexa-Fluor-647-donkey anti-goat | Jackson ImmunoResearch Labs | Cat# 705-605-147, RRID:AB_2340437 |
| Alexa-Fluor-750-donkey anti-rabbit | Abcam | Cat. # ab175731; RRID:AB_2943056 |
|
| ||
| Bacterial and virus strains Titer | ||
|
| ||
| AAV6-Syn-ChR2-eYFP | ViroVek | 2-5e+13 vg/ml |
| HSV-hEF1 α-LS1L-GCaMP6s | Massachusetts General Hospital | 5 × 109 vg/ml |
| AAV5-ef1 α-DIO-ChR2-eYFP | UNC Vector Core | 5 ×1012 vg/ml |
| AAV5- ef1 α -DIO-eYFP-WPRE-PA | UNC Vector Core | 5 ×1012 vg/ml |
| AAV5-CaMKII-ChR2(H134R)-mCherry | UNC Vector Core | 5 ×1012 vg/ml |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| VGaT-IRES-Cre | The Jackson Laboratory | Stock#: 016962; RRID:IMSR_JAX:016962 |
| Vglut2-IRES-Cre | The Jackson Laboratory | Stock #: 016963, backcrossed onto C57Bl/6 at NIDA IRP; RRID:IMSR_JAX:016963 |
| TH-Cre | International Mouse Strain Resource | MGI: 3056580 |
|
| ||
| Software and algorithms | ||
|
| ||
| Synapse | Tucker Davis Technologies | RRID:SCR_006495 |
| Med PC IV | Med-Associates | N/A |
| MATLAB Scripts | MathWorks |
https://github.com/djamesbarker/FiberPhotometry
https://doi.org/10.5281/zenodo.5781801 |
| Prism 8.0 & 9.0 | GraphPad | RRID: SCR_002798 |
| Adobe Creative Cloud | Adobe | RRID: SCR_014199 |