Table 8. Summary of defects in shmoo formation among HSP70 and HSP90 network mutants.
| Strain1 | aF mM | %Shmoo | # shmoo2 | #total-shmoo2 p-value3 | |
|---|---|---|---|---|---|
| WT EY3136 | 5 | 54 (100)4 | 169 | 146 | |
| ssa1D ssa2D EY3141 | 5 | 15 (28) | 60 | 342 | <0.00001 |
| 1D2D4D GAL1p-SSA1 | 5 | ||||
| t = 0 hr repress SSA1 | 34.6 (100) | 121 | 229 | ||
| t = 6 hr repress SSA1 | 3.1 (9) | 11 | 339 | <0.00001 | |
| WT EYL1740 | 2.5 | 36 (100)2.5 | 174 | 309 | |
| WT EYL1740 | 5 | 54 (100)5 | 512 | 432 | |
| snl1D | 2.5 | 24 (67) | 238 | 542 | 0.0481 |
| fes1D | 2.5 | 27 (75) | 273 | 726 | 0.1539 |
| fes1D | 2.5 | 34 (94) | 386 | 761 | 0.3613 |
| fes1D | 2.5 | 31 (86) | 659 | 1487 | 0.0264 |
| fes1D total tally | 2.5 | 28.7 (80) | 1318 | 2974 | 0.0197 |
| sti1D | 5 | 82 (152) | 378 | 84 | <0.00001 |
| ydj1D | 2.5 | 24 (67) | 133 | 416 | 0 |
| ydj1D | 5 | 23 (45) | 110 | 172 | <0.00001 |
| ssb1D | 5 | 46 (85) | 241 | 245 | 0.1049 |
| ssb1D 5 | 5 | 53 (98) | 336 | 633 | <0.00001 |
| sse1D | 5 | 42 (78) | 262 | 368 | <0.00001 |
| sse2D | 5 | 0 (0) | 0 | 202 | <0.00001 |
| ssz1D | 5 | 8 (15) | 54 | 614 | <0.00001 |
2#Shmoo is the number of cells that had a shmoo morphology. #Total-shmoo is the total number of cells scored minus the number of shmoo cells.
3p-values were calculated using the Fisher’s Exact Test at the Social Science Statistics website socistatistics.com, copyright 2023 Jeremy Stangroom.
4The numbers in parentheses (#) are % wild type shmoo formation.
5The sample was counted a second time with more cells in the tally.