Fig. 3. BCRP+ cancer cells can undergo VM in BCBM.
(A) Schematic representation of various canal-like structures found in BCBM, and assessed in the rest of the figure via IHC. (B and C) Representative images and quantification of IHC dual staining of BCRP (blue) and CK8 (brown) in the BCBM of (B) the PyMT#1 model and (C) the KB1P model. (D and E) Dual staining and quantification of BCRP (blue) and CD31 (brown) in the BCBM of (D) the PyMT#1 model and (E) the KB1P model. Gray arrows in (B) to (E) indicate single positive staining, and orange arrows indicate double positive staining. (F and G) Representative images of IHC dual staining of PAS (pink) and CD31 (orange) in the BCBM of (F) the PyMT#1 model and (G) the KB1P model. (H) Representative images of IHC staining of TER-119 in the BCBM of the PyMT#1 model and of the KB1P model. Dotted lines in (H) indicate the lumen of the canal-like structures. (I and J) Representative images of IHC staining of BCRP in organoids derived from the BCBMs in (I) the PyMT#1 model and (J) the KB1P model. For this figure, data were analyzed in three mice per group.