Extended Data Fig. 10. Treatment with BAI1 improves healthspan and reduces inflammation but it does not affect lifespan of aged mice.
(a) Neuromuscular coordination shown as a percentage number of successful attempts (green) to remain on a straight rod for 60 s (n = 7 vehicle and n = 8 BAI1-treated mice). (b) Kaplan-Meier survival curves of animals treated with vehicle (n = 38) or Bax inhibitor (n = 39) from 18–20 months old until death. (c) Heatmap showing levels of cytokines found in plasma from mice treated with vehicle or BAI1. Values are shown as fold change compared to vehicle-treated animals. Red denotes high expression and blue indicates low expression. d) Table summarizing μCT-derived parameters obtained for the spine and femur from vehicle (n = 6) and BAI1-treated mice (n = 10). e) mRNA expression of p16, p21 and p53 in control (n = 6) and BAI-1 treated mice (n = 10). f) (left) Heatmap showing mRNA expression of the indicated SASP factors in the brain from aged animals treated with BAI1 (p = 0.04). (right) Graphs showing quantification of levels of mRNA of the indicated genes in brains from aged mice treated with BAI1 (n = 8 vehicle- and n = 7 BAI1-treated mice). Data are mean ± S.E.M. Statistical significance was assessed using two-sided Student’s unpaired t-test (d, f), MANOVA (heatmap in f).