Extended Data Fig. 3. BAX and BAK deletion inhibits the SASP in therapy-induced senescent cells.

Quantification of mRNA expression levels of (a) p16^INK4A, (b) p21 and SASP factors (c) IL-1α, (d) IL-1β, (e) IL-6 and (f) IL-8 in proliferating and doxorubicin-induced senescent (n = 3 independent experiments) BAX/BAK^−/− MRC5 human fibroblasts. Quantification of mRNA levels of (g) p16^INK4A, (h) p21, (i) IL-1 α, (j) IL-1β, (k) IL-6 and (l) IL-8 in proliferating and etoposide-induced senescent (n = 3 independent experiments) BAX/BAK^−/− MRC5 human fibroblasts. Data are expressed as fold change to proliferating Empty^CRISPR cells. Data are mean ± S.E.M. Statistical significance was assessed using one-way ANOVA followed by Tukey’s multiple comparison test (a-l).

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