. 2023 Aug 28;30(10):1446–1455. doi: 10.1038/s41594-023-01070-z

Table 1.

Summary of NMR-based dynamics results for WT, G12C and G12D K-Ras bound to GTP at 298 K. Uncertainties in the parameters were determined through bootstrap analysis

Sample		¹⁵N CPMG^a	¹H^N CPMG^a	¹⁵N CEST^a	k₂₁ (s⁻¹)	k₁₂ (s⁻¹)	k_ex (s⁻¹)^b	p₁ (%)^c
GTP	WT	29	22	28	40.6 ± 2.2	359 ± 11	400 ± 12	10.15 ± 0.47
	G12C	31	–	31	22.7 ± 0.9	303 ± 10	326 ± 11	6.97 ± 0.17
	G12D	50	22	50	27.1 ± 2.0	274 ± 16	301 ± 17	9.00 ± 0.44

^aTotal number of residues with significant conformational exchange contributions that were included in parametrization of two-site exchange model by global nonlinear least squares fitting.

^bGlobally fitted exchange rate constant k_ex = k₂₁ + k₁₂ using a two-state conformational exchange model consisting of a ground state (state 2) and an excited state (state 1).

^cGlobally fitted population p₁ of the excited state (state 1), whereby p₁ = 1 – p₂.