TABLE 1.
Effect of early or delayed treatment with a single dose of PEG-AMB-LIP versus single- or multidose AmBisome on survival of leukopenic micea and growth of C. albicans in the kidney
| Time of start of treatment (h postinoculation) | Treatmentb | Daily dose (mg/kg) | No. of doses | Log10 CFU/kidney in surviving mice on dayc:
|
||
|---|---|---|---|---|---|---|
| 0 | 3 | 7 | ||||
| 6 | None | 3.20 ± 0.16 | ||||
| Placebo | 5.94 ± 0.11 (50) | 6.86 ± 0.07 (30) | ||||
| PEG-AMB-LIP | 5 | 1 | 1.04 ± 0.39d (100 | 1.16 ± 0.70d (100) | ||
| AmBisome | 5 | 1 | 3.28 ± 0.20 (100) | 3.47 ± 0.41 (100) | ||
| AmBisome | 5 | 3 | 3.08 ± 0.26 (100) | 3.46 ± 0.57 (100) | ||
| AmBisome | 5 | 5 | 2.90 ± 0.44 (100) | 2.63 ± 0.46d (100) | ||
| 16 | None | 4.01 ± 0.22 | ||||
| PEG-AMB-LIP | 5 | 1 | 2.29 ± 0.42d (100) | 1.35 ± 0.51d,e (100) | ||
| AmBisome | 5 | 5 | 4.79 ± 0.40 (90) | 3.43 ± 0.38 (90) | ||
| 20 | None | 4.34 ± 0.19 | ||||
| PEG-AMB-LIP | 5 | 1 | 2.88 ± 0.38d (100) | 2.31 ± 0.19d,e (100) | ||
| AmBisome | 5 | 5 | 5.39 ± 0.45 (60) | 4.57 ± 1.11 (30) | ||
Leukopenic mice were inoculated i.v. at time zero with 3 × 104 CFU of C. albicans; untreated mice died between 24 h and 8 days after C. albicans inoculation.
PEG-AMB-LIP, AmBisome, and the placebo were administered i.v.
The effect of treatment was determined at day 3 (n = 10) as well as day 7 (n = 10) after inoculation. Each value represents the geometric mean ± the standard deviation. The numbers in parentheses indicate the percentages of surviving mice. Values at day 0 are log10 CFU/kidney at the start of treatment.
P ≤ 0.002 compared with the number of CFU at the start of treatment.
P ≤ 0.002 compared with the number of CFU at day 3 after inoculation.