Fig. 4. 2-AG dMSN signaling deletion results in decreased ethanol preference and increased water consumption in males.

Individual data points are overlaid as circles, with DGLα ^flx/flx in gray and DGLα ^{D1 Cre+} in red. a Schematic of 16 h intermittent ethanol 2-bottle choice drinking paradigm. Ethanol intake in males (b) and females (c) across 16 h of intermittent drinking. Preference for ethanol, consumption of water, and total fluids in males (d–f) and females (g–i) in DGLα ^flx/flx and DGLα ^{D1 Cre+} mice. Littermate controls, DGLα ^flx/flx, represented as gray bars and DGLα ^{D1 Cre+} mice represented as red bars. Individual data points for females shown as pink circles and males as blue circles for each genotype. j Schematic of experimental procedure. k Water consumption following a challenge injection of ethanol. l Schematic of water restriction experimental procedure. m Water consumption following 16 h of water restriction. Data presented as mean ± SEM. ***p < 0.001.