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. 2023 Oct 18;13:17803. doi: 10.1038/s41598-023-45017-2

Figure 5.

Figure 5

CGA pretreatment protects HL-1 cardiomyocytes against H/R injury by inhibiting pyroptosis. (a,b) HL-1 cardiomyocytes were pretreated with 0.2 μM CGA or 2 μM CGA for 12 h before H4R4. Effect of CGA on the protein expression levels of pro-caspase-1 and cleaved-caspase-1 in HL-1 cardiomyocytes. The protein expression levels of pro-caspase-1 and cleaved-caspase-1 were measuring by Western blotting analysis. (c–e) Effect of CGA on the protein expression levels of GSDMD, N-terminal GSDMD, IL-1β and IL-18 in HL-1 cardiomyocytes. The protein expression levels of GSDMD, cleaved N-terminal GSDMD, IL-1β and IL-18 were measuring by Western blotting analysis. (f,g) CGA pretreatment decreased the H/R injury-induced rate of positive PI staining in HL-1 cardiomyocytes. Representative images were captured using a fluorescence microscope (×100  magnification). The data are expressed as the mean ± SD. n = 3 independent experiments. The full-length blots are presented in Supplementary Fig. 4. *P < 0.05 vs. the control group, #P < 0.05 vs. the H4R4 group.