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. 2023 Oct 18;13:17803. doi: 10.1038/s41598-023-45017-2

Figure 6.

Figure 6

Inhibition of Lnc Neat1 reduces NLRP3 inflammasome activation and pyroptosis induced by H/R injury in HL-1 cardiomyocytes. (a) Effect of knocking down Lnc Neat1 on the mRNA expression levels of NLRP3 and ASC in HL-1 cardiomyocytes. The mRNA expression levels of NLRP3 and ASC were detected by qRT-PCR analysis. (b–d) Silencing Lnc Neat1 reversed H/R injury-induced the protein expression levels of NLRP3 and ASC oligomerization. (e,f) Effect of knocking down Lnc Neat1 on the protein expression levels of pro-caspase-1 and cleaved-caspase-1 in HL-1 cardiomyocytes. The protein expression levels of pro-caspase-1 and cleaved-caspase-1 were detected by Western blot analysis. (g–i) Knockdown of Lnc Neat1 expression attenuated the protein expression levels of cleaved N-terminal GSDMD, IL-1β and IL-18 after H/R. The protein expression levels of GSDMD、cleaved N-terminal GSDMD、IL-1β and IL-18 were detected by Western blot analysis. (j,k) Knocking down Lnc Neat1 expression reduced the positive rate of PI staining in HL-1 cardiomyocytes subjected to H/R injury. Representative images were captured using a fluorescence microscope (100 × magnification). Data are expressed as the mean ± SD. n = 3 independent experiments. The full-length blots are presented in Supplementary Fig. 5. *P < 0.05 vs. contorl group, #P < 0.05 vs. H4R4 group, ▲P < 0.05.