Table 4.

Six-factor D-optimal quadratic response surface design matrix. The design included 33 model points, 5 lack-of-fit points, and 5 replicated points for pure error estimation.

ID*	Block	Design points (runs)	Factor 1	Factor 2	Factor 3	Factor 4	Factor 5	Factor 6
			BAP	Tween 20	DMSO	Solvent	Orientation	Light type
			mM	%	%	Type	Direction	Type
9	1	1	0	0	5	EtOH	North	MH
11	1	2	5	5	0	Water	South	MH
3	1	3	0	0	0	EtOH	South	None
7	1	4	5	5	0	EtOH	South	LED
7	1	5	5	5	0	EtOH	South	LED
10	1	6	5	5	5	EtOH	South	MH
0	1	7	2.5	2.5	2.5	Water	North	LED
11	1	8	5	5	0	Water	South	MH
1	1	9	0	5	0	EtOH	North	None
8	1	10	0	5	5	Water	South	LED
5	1	11	5	0	5	Water	South	None
2	1	12	2.5	5	5	Water	North	None
4	1	13	2.5	3.75	2.5	EtOH	South	None
10	1	14	5	5	5	EtOH	South	MH
12	1	15	0	0	2.5	Water	South	MH
6	1	16	5	0	5	EtOH	North	LED
24	2	17	0	5	0	EtOH	South	MH
21	2	18	0	0	0	Water	North	MH
15	2	19	0	5	0	Water	South	None
14	2	20	5	2.5	5	EtOH	North	None
13	2	21	5	5	2.5	EtOH	North	None
18	2	22	2.5	0	5	EtOH	South	LED
20	2	23	5	5	0	EtOH	North	MH
17	2	24	0	5	5	EtOH	North	LED
22	2	25	5	0	5	Water	North	MH
24	2	26	0	5	0	EtOH	South	MH
25	2	27	2.5	2.5	5	Water	South	MH
22	2	28	5	0	5	Water	North	MH
16	2	29	0	0	0	EtOH	North	LED
19	2	30	5	0	2.5	Water	South	LED
23	2	31	5	0	0	EtOH	South	MH
27	3	32	5	0	0	Water	North	None
28	3	33	0	0	5	Water	North	None
31	3	34	2.5	1.25	1.25	Water	South	None
32	3	35	0	5	0	Water	North	LED
35	3	36	0	0	0	Water	South	LED
30	3	37	0	5	5	EtOH	South	None
36	3	38	1.25	2.5	1.25	EtOH	North	MH
37	3	39	0	5	5	Water	North	MH
29	3	40	5	5	0	EtOH	South	None
34	3	41	0	2.5	2.5	EtOH	South	LED
26	3	42	2.5	0	2.5	EtOH	North	None
33	3	43	5	5	5	Water	North	LED

*Unique treatment identifier. For example, run #2 and run #8 are replicates and have the same ID of #11.