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. 2023 Oct 18;10:713. doi: 10.1038/s41597-023-02631-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Distribution of raw reads from long read sequencing and genome assembly. (A) Read distributions from each sequencing batch. The x-axis indicates Nanopore sequencing batch, and the y-axis indicates read length. M, MinION; P, PromethION; S, sheared; N, non-sheared; F20, 20 kb size fractionation; F40, 40 kb size fractionation. (B) Read length ratio for each sequencing batch. The x-axis indicates ratio of read length, and the y-axis indicates sequencing batch. (C) Read N50 values for each sequencing batch. (D,E) Genome assembly improvements by Purge Haplotigs with ONT (D) and PacBio (E). The x-axis indicates value of parameters, and the y-axis indicates genome quality parameters.