Figure 4.

Germinal center reactions. GC reaction in lymph nodes of mice, immunized as described in the legend of Figure 1 , were assessed by measuring the frequency (A) and absolute number (B) of CD19⁺GL7⁺ B_GC cells, the frequencies of CD19⁺GL7⁺CD86⁺ light zone B_GC cells (C), CD19⁺GL7⁺CXCR4⁺ dark zone B_GC cells (D), and ratio of light zone B cells/dark zone B cells (E), CD3⁺CD4⁺PD1⁺CXCR5⁺ T_FH cells (F), and CD19⁺GL7⁺IgD^-CD138⁺plasmablasts (G). Statistical comparisons between experimental groups were done using one-way ANOVA with Tukey post-hoc test (* p < 0.05, ** p < 0.01, *** p < 0.001). B_GC, germinal center B; DZ, dark zone; GC, germinal center; T_FH, Follicular T helper; LZ, Light zone; ns, not significantly different.