Figure 4.

Binding of the Fc-fusion proteins to murine FcγRs. Four-fold serial dilutions of the purified and biotinylated gp120 – Fc (A) and RBD – Fc (B) fusion proteins were incubated with recombinant murine FcγRs in an ELISA-based assay. Binding of Fc-fusion proteins was detected by HRP coupled streptavidin. Mean of duplicates and SD are displayed on each graph. One representative out of two independent experiments is shown. The statistical significance of observed differences of gp120 – Fc WT and RBD – Fc WT to the mutants was calculated for each concentration by One-Way ANOVA followed by Tukey’s post test; p-values indicate significant differences (* p<0.05; ** p<0.005; *** p<0.0005).