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. 2023 Oct 5;14:1275193. doi: 10.3389/fimmu.2023.1275193

Figure 8.

Figure 8

RBD-specific CD4 and CD8 T cell responses after immunization with RBD – Fc fusion proteins. Splenocytes from immunized mice at week 5 were isolated and stimulated for 6h with a RBD peptide pool in presence of Brefaldin A and a CD28 antibody. CD4+ (A) and CD8+ (B) T-cells expressing TNFα, IL-2 and IFNγ were analyzed by FACS following an intracellular cytokines staining. (C, D) Splenocytes were stimulated with a RBD peptide pool in presence of CD28 antibody for 48h. Cell supernatants were harvested, diluted 1:3 and IL-4 (C), IL-5 (D) and IL-10 (E) concentrations were determined by quantitative ELISAs. The cut-off value is indicated by the dotted line. Bars represent mean and SD of each group overlaid with individual data point (n=6 per group). Significant differences as determined by One-Way ANOVA followed by Tukey’s multiple comparison test are indicated (* p<0.05; ** p<0.005; *** p<0.005; **** p<0.0001).