Figure 4.

Transcriptome of granulomatous yeasts indicates upregulation of siderophores biosynthesis and downregulation of metabolism and cell cycle. Granulomatous lesions were extracted from two to four mice from three independent infections at eight weeks (8W) and twelve weeks (12W) after being infected with 1 x 10⁶ P. brasiliensis yeasts. Then, extraction of total RNA, removal of rRNA, and assembly of cDNA libraries were performed. The samples were sequenced and the fragments aligned with the reference genome for P. brasiliensis using bowtie2. The FeatureCounts software was used to count the amount of each identified gene, which were normalized. Then, a differentially expressed genes analysis was made using limma and a functional analysis was carried out. To illustrate the differentially expressed genes, a volcano plot of the 8-weeks infection (8W) group in relation to the control yeasts (A) and a volcano plot of the 12-weeks infection group (12W) in relation to the control yeasts (B) were made. Additionally, a heatmap was created with selected genes (C). The genes in red represent a higher expression and the green ones a lower expression for a given sample. The functions of the upregulated genes (D) and downregulated genes (E) were taken using GO, KEGG and FungiDB.