Table 2.
Differential expression of yeasts genes.
| Acession number | Protein | Expression status | Log (Fold Change) | Adjusted p-value |
|---|---|---|---|---|
| UPs | ||||
| Siderophore synthesis and transport | ||||
| PADG_00097 | L-ornithine N5-oxygenase SidA | UP(D) | 3,594664 | 2,32E-08 |
| PADG_00095 | MFS siderochrome iron transporter 1 | UP(D) | 3,188131 | 1,12E-08 |
| PADG_00100 | Hydroxyornithine transacylase SIDF | UP(D) | 2,354155 | 3,4E-06 |
| PADG_00102 | fusarinine C synthase | UP(D) | 2,227774 | 5,26E-07 |
| PADG_00104 | Oxidoreductase OXR1 | UP(D) | 1,957033 | 4,87E-05 |
| PADG_00099 | Acyl-CoA ligase SID4 | UP(D) | 1,406269 | 8,21E-05 |
| Virulence factors | ||||
| PADG_02842 | Superoxide dismutase, Cu-Zn | UP(D) | 1,424825 | 3,48E-05 |
| PADG_04701 | alcohol dehydrogenase [EC:1.1.1.-] | UP(8) | 1,042112 | 1,75E-06 |
| PADG_04274 | polysaccharide synthase Cps1 | UP(8) | 1,027865 | 0,001103 |
| Amino acid metabolis | ||||
| PADG_07440 | Amino acid permease | UP(D) | 1,812242 | 8,64E-07 |
| PADG_08406 | O-acetylhomoserine/O-acetylserine sulfhydrylase [EC:2.5.1.49 2.5.1.47] | UP(D) | 1,637297 | 0,000873 |
| PADG_00215 | aromatic-L-amino-acid/L-tryptophan decarboxylase [EC:4.1.1.28 4.1.1.105] | UP(D) | 1,514618 | 0,000419 |
| PADG_02214 | 4-aminobutyrate aminotransferase/(S)-3-amino-2-methylpropionate transaminase [EC:2.6.1.19 2.6.1.22] | UP(8) | 1,140801 | 4,12E-06 |
| Other metabolisms | ||||
| PADG_06196 | Flavin oxidoreductase hxnT | UP(D) | 1,98727 | 4,73E-06 |
| PADG_03085 | Mannose-P-dolichol utilization defect 1 protein homolog | UP(12) | 1,076101 | 0,000244 |
| PADG_05433 | pyridoxamine 5’-phosphate oxidase [EC:1.4.3.5] | UP(8) | 1,032033 | 5,7E-05 |
| DOWNs | ||||
| Gene/protein regulation | ||||
| PADG_04314 | Zn(2)-C6 fungal-type domain-containing protein | DOWN(8) | -1,24281 | 4,13E-05 |
| PADG_04889 | Zn(2)-C6 fungal-type domain-containing protein | DOWN(D) | -1,23166 | 0,002813 |
| PADG_05497 | GATA-binding protein, other eukaryote | DOWN(D) | -1,22538 | 0,002127 |
| PADG_02195 | DNA repair protein Swi5/Sae3 | DOWN(8) | -1,21794 | 0,00134 |
| PADG_04429 | Structure-specific endonuclease subunit SLX4 | DOWN(8) | -1,14817 | 0,00099 |
| PADG_00411 | C6 transcription factor (Ctf1B) | DOWN(D) | -1,05195 | 0,00555 |
| PADG_06413 | C6 finger domain transcription factor nscR | DOWN(8) | -1,00975 | 0,008026 |
| Energy metabolism | ||||
| PADG_11844 | aconitate hydratase | DOWN(D) | -1,52072 | 0,01042 |
| PADG_00393 | hexokinase [EC:2.7.1.1] | DOWN(12) | -1,38879 | 0,000134 |
| PADG_03602 | acyl-CoA dehydrogenase | DOWN(D) | -1,32085 | 0,000697 |
| PADG_05290 | Cytochrome b5 heme-binding domain-containing protein | DOWN(D) | -1,07453 | 0,005483 |
| Cell cylce | ||||
| PADG_03022 | PLK/PLK1 protein kinase | DOWN(D) | -1,25446 | 0,002207 |
| PADG_11709 | serine/threonine-protein kinase TTK/MPS1 [EC:2.7.12.1] | DOWN(D) | -1,23479 | 5,54E-05 |
| Virulence factors | ||||
| PADG_01954 | superoxide dismutase, Fe-Mn family [EC:1.15.1.1] | DOWN(D) | -2,55152 | 5,85E-06 |
| PADG_05660 | Fungal nitric oxide reductase | DOWN(D) | -1,38287 | 0,007157 |
| PADG_01716 | alcohol dehydrogenase zinc-binding | DOWN(D) | -1,35725 | 0,000574 |
| Other metabolisms | ||||
| PADG_02384 | porphobilinogen synthase [EC:4.2.1.24] | DOWN(D) | -1,38467 | 0,000981 |
| PADG_07849 | N5-hydroxyornithine acetyltransferase [EC:2.3.1.-] | DOWN(12) | -1,12162 | 0,005081 |
| PADG_04432 | alpha-amylase [EC:3.2.1.1] | DOWN(D) | -1,06127 | 0,002921 |
Granulomatous lesions were extracted from two to four mice from three independent infections at eight weeks and twelve weeks after being infected with 1x106 P. brasiliensis yeasts. Then, extraction of total RNA, removal of rRNA, and assembly of cDNA libraries were performed. The samples were sequenced and the fragments aligned with the reference genome for P. brasiliensis using bowtie2. The FeatureCounts software was used to count the amount of each identified gene, which were normalized. Then, a differentially expressed gene was made using the limma and a functional analysis was carried out using GO, KEGG and FungiDB. The Table shows the differentially expressed genes with the values of Log (Fold change) and adjusted p-value. The expression status column represents the upregulated or downregulated genes for the eight-week infection group (8), the twelve-week infection group (12), and the genes that were differentially expressed in both infection groups were combined and reanalyzed, resulting in an expression status designation for the disease (D).