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. 2023 Oct 5;9(10):e20713. doi: 10.1016/j.heliyon.2023.e20713

Fig. 3.

Fig. 3

Assessment of differentially expressed genes (DEGs) induced by adipogenic induction in 2D and 3D culture conditions in the 3T3-L1 preadipocytes using RNA sequencing.

DEGs induced by adipogenic induction in the 2D and 3D culture conditions of 3T3-L1 preadipocytes and are shown by hierarchical clustering heatmaps (2D: panel A, 3D: panel D), Minus-Average (M–A) plots of the relationship between the mean expression values [log (baseMean); x-axis] and the magnitude of change in gene expression (log2 of fold-change; y-axis) (2D: panel B, 3D: panel E) and volcano plots of the relationship between magnitude of gene expression change (log2 of fold-change; x-axis) and statistical significance of the change [−log10 of false discovery rate (FDR); y-axis] (2D: panel C, 3D: panel E). Red and blue points represent significantly over-expressed or under-expressed DEGs (cutoff FDR <0.05 and/or magnitude of change ≥2) upon adipogenic induction, respectively. DIF-: undifferentiated cells. DIF+: differentiated cells.