Fig. 4.

Quantitative RT-PCR analysis of selected genes among the top 10 up-regulated DEGs upon adipogenic differentiation in the 2D and 3D cultured 3T3-L1 preadipocytes. 2D and 3D cultures of 3T3-L1 preadipocyte (NT) and their adipogenesis forms (DIF) at Day 6 were subjected to qPCR analysis of Adig and Mrap, or Cd36, Adiponectin and Cidec which were identified as predominantly upregulated in the 2D culture condition or commonly upregulated in 2D and 3D culture conditions, respectively. All experiments were repeated twice using fresh preparations consisting of 16 spheroids for each qPCR analysis. Data are presented as means ± standard error of the mean (SEM). ****P < 0.001 (ANOVA followed by Tukey's multiple comparison test).