Fig. 8.

Differentially expressed genes (DEGs) for differentiated 3T3-L1 cells between 2D and 3D cultures.

Panel A: DEGs of DIF+ 3T3-L1 cells between 2D and 3D cultures are shown by a volcano plot. Among the significantly up-regulated DEGs, several mitochondria-related genes were detected. Panel B: 2D and 3D cultures of 3T3-L1 preadipocytes (NT) and their adipogenesis forms (DIF) at Day 7 were subjected to quantitative RT-PCR analysis of selected mitochondrial factors, in which there was a significant correlation with adipocyte metabolism: mt-Nd6 (complex I), mt-Cytb (complex III) and mt-Co1 (complex IV). All experiments were conducted using fresh preparations consisting of 16 spheroids each for qPCR analysis. Data are presented as means ± standard error of the mean (SEM). ****P < 0.001 (ANOVA followed by Tukey's multiple comparison test).