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. 2023 Oct 5;26(11):108133. doi: 10.1016/j.isci.2023.108133

Figure 7.

Figure 7

Confirmation of plasma cells in ILD lungs by scRNAseq

(A) UMAP atlas of scRNAseq from explanted lung cells depicted an MZB1 expressing subset. The annotated subsets of SSc-ILD lung cells are shown on the left (a) as the reference for cell type position on the UMAP atlas and MZB1 positive (b) and MS4A1 (encoding CD20) (c) cell subsets are depicted with a color spectrum. AEC: alveolar epithelial cells; FB: fibroblasts; SMC: smooth muscle cells; Endo: endothelium; UD: undetermined cells; PC1/PC2, plasma cells.

(B) Skin PC signature genes are distinctly expressed in plasma cells in SSc-ILD lung tissue. The gene expression of interest and relevant to B cell development stages are presented in a stacked violin plot in comparison between the control and SSc, where genes related to specific B cell sub-types are listed on the right of y axis and the annotated clusters on x axis. The colors represent the sources of RNAseq, blue from SSc-ILD lung (SSc-ILD), whereas gray from the healthy control (HC). TNFRSF17: encoding BCMA; TNFRSF13B: encoding TACI.