Figure 7.

Separate knockdown of H6PD and G6PD has differential effects on 2-[¹⁸F]FDG accumulation and metabolism

(A) WB of HT29 cells treated with siRNA for the respective enzyme or non-targeting siRNA (NTsi) (samples from different experiments).

(B and C) Effects of G6PD/H6PD knockdown on 2-[¹⁸F]FDG accumulation (B) and metabolism (n = 4 each) (C), the box showing relevant radiometabolite changes. % AD = % applied dose. Asterisks mark significant differences between knockdown groups (H6PDsi or G6PDsi) and NTsi (control) (unpaired t-test, ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001). Data are represented as mean ± SD.