REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-G6PD	Abcam	Cat# ab210702; RRID:AB_2923527
anti-H6PD	Abcam	Cat# ab170895; RRID:AB_2864314
anti-H6PD	Proteintech	Cat# 15255-1-AP; RRID:AB_10642699
anti-β-actin	Abcam	Cat# ab8227; RRID:AB_2305186
Goat anti-Rabbit IgG	ThermoFisher	Cat# A16104; RRID:AB_2534776
Chemicals, peptides, and recombinant proteins
2-[18F]FDG	in-house production, formulated for patient use	N/A
Matrigel	Sigma-Aldrich	Cat# E6909
Modified Eagle Medium	GibcoTM	Cat# 10370-047
Roswell Park Memorial Medium	GibcoTM	Cat# 21875-034
L-glutamine	GibcoTM	Cat# 25030-024
Fetal bovine serum	GibcoTM	Cat# 10270-106
Opti-MEM	GibcoTM	Cat# 31985070
DharmaFECT transfection reagent 1	Horizon	Cat# T-2001-03
In vivo-jetPEI®	Polyplus	Cat# 101000040
Dehydroepiandrosterone	Sigma-Aldrich	Cat# D4000
Carbenoxolone	Sigma-Aldrich	Cat# C4790
G6PDi-1	Kindly provided by the Rabinowitz-Lab	Cat# SML2980
Glucose-6-phosphate	Sigma-Aldrich	Cat# G7879
2-fluoro-2-deoxy-D-glucose-6-phosphate	Biosynth® Carbosynth	Cat# MD94505
Critical commercial assays
Pierce™ BCA Protein Assay Kit	ThermoFisher	Cat# 23225
Experimental models: Cell lines
HT1080 cell line	ATCC	CCL-121™; RRID:CVCL_0317
HT29 cell line	ATCC	HTB-38™; RRID:CVCL_0320
Experimental models: Organisms/strains
Fox Chase SCID Beige mice (female)	Charles River	CB17.Cg-PrkdcscidLystbg-J/Crl; RRID:IMSR_CRL:250
Oligonucleotides
ON-TARGETplus siRNA pool G6PD ACAGAUACAAGAACGUGAA, CCGUGUA CACCAAGAUGAU, CAGAUAGGCUGGAA CCGCA, AUUCACGAGUCCUGCAUGA	Horizon	Cat# L-008181-02-0005
ON-TARGETplus siRNA pool H6PD CGUCUGUUAUAAAGCGUUA, CGUGGUGG GCUGAGGUUAA, GCGGGUUGUCCUUGA GAAA, UGGACGAGAGAGUGGGCUA	Horizon	Cat# L-004692-01-0005
ON-TARGETplus siRNA pool non-targeting CCGCAGGGCUCAUGAGUAU, GGACAAACA CCCAUGAACA, AGGAACAAACGUUGACUUA, CCAAAUCUCGUGAUGAAUC	Horizon	Cat# D-001810-10-20
G6PD siRNA “No. 19” (in vivo grade) CCGUGUACACCAAGAUGAU	Horizon	Cat# CTM-733527
G6PD siRNA “No. 20” (in vivo grade) CAGAUAGGCUGGAACCGCA	Horizon	Cat# CTM-733528
H6PD siRNA “No. 10” (in vivo grade) CGUGGUGGGCUGAGGUUAA	Horizon	Cat# CTM-733521
H6PD siRNA “No. 11” (in vivo grade) GCGGGUUGUCCUUGAGAAA	Horizon	Cat# CTM-733525
Non-targeting siRNA (in vivo grade) GGACAAAC ACCCAUGAACA	Horizon	in vivo grade Cat# J-016083-06
Cy5.5 labeled non-targeting siRNA (in vivo grade) GGACAAACACCCAUGAACA	Horizon	Cy5.5 labeled in vivo grade Cat# J-016083-06
Software and algorithms
PMOD	PMOD Technologies LLC	Version 3.8; RRID:SCR_016547
Fiji	N/A	RRID:SCR_002285
GraphPad Prism	GraphPad	Version 7.03, RRID:SCR_002798
SPSS statistics	IBM	Version 27, RRID:SCR_019096
Gina X HPLC software	Elysia Raytest	Version 10.4
Other
Partisil™ 10 SAX column (250 mm × 4.6 mm)	Supelco Analytical	Cat# 50193-U
Wizard23 2480 automatic gamma counter	PerkinElmer	N/A
Inveon® μPET/SPECT/CT	Siemens	N/A
HPLC system	Shimadzu	N/A
Ramona∗ radioactivity-HPLC flow detector	Elysia Raytest	N/A