Figure 1.

Identification ALDH1A1 with lysine 2-hydroxyisobutyrylation by MS from bladder cancer

(A) Differential Khib modification sites and proteins respectively between bladder cancer tissues and adjacent normal tissues (>1.2 or <0.83) (n = 10 per group).

(B) Flanking sequence analysis of all Khib sites.

(C) The subcellular localization of proteins.

(D) GO enrichment of differentially expressed proteins.

(E) KEGG analysis of proteins with the different Khib modification levels.

(F) Increased expression of ALDH1A1 was associated with advanced tumor stage in TCGA-BLCA database.

(G) Kaplan-Meier survival curves showed that higher ALDH1A1expression was significantly related with worse OS in TCGA-BLCA database.

(H) Higher expression of ALDH1A1 was related with advanced tumor stage in 105 patients with bladder cancer from our hospital (I) Kaplan-Meier survival curves revealed that higher ALDH1A1 expression was significantly related with worse OS in 105 patients with bladder cancer from our hospital.

(J‒L) The correlation of ALDH1A1 with CD44, CD133, and CD47 in the TCGA-BLCA database.