REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-ALDH1A1	Proteintech	Cat #: 15910; RRID:AB_2305276
Mouse monoclonal anti-ALDH1A1	Proteintech	Cat #: 60171; RRID:AB_10693634
Rabbit polyclonal anti-AKT	Cell Signaling	Cat #: 9272; RRID:AB_329827
Rabbit polyclonal anti-Phospho-Akt	Cell Signaling	Cat #:4060; RRID:AB_2315049
Mouse polyclonal anti-CD44	Cell Signaling	Cat#:5640; RRID:AB_AB_10547133
Mouse monoclonal anti-HDAC3	Cell Signaling	Cat #:3949; RRID:AB_2118371
Rabbit polyclonal anti-HDAC3	Proteintech	Cat #:10255; RRID:AB_2279733
Rabbit polyclonal anti-HDAC2	Proteintech	Cat #:12922; RRID:AB_2934877
Mouse monoclonal anti-HDAC2	Proteintech	Cat #:67165; RRID:AB_2920291
Rabbit monoclonal anti-Flag-Tag	Cell Signaling	Cat #:14793; RRID:AB_2572291
Rabbit monoclonal anti-HA-Tag	Cell Signaling	Cat #:3724; RRID:AB_1549585
Mouse monoclonal anti-SQSTM1/p62	Cell Signaling	Cat #:88588; RRID:AB_2800125
Rabbit monoclonal anti-LC3	Cell Signaling	Cat #:3868; RRID:AB_2137707
Mouse monoclonal anti-β-catenin	Cell Signaling	Cat #:2698; RRID:AB_1030945
Mouse monoclonal Anti-2-Hydroxyisobutyryllysine	PTMBIO	Cat #:PTM802;
Rabbit polyclonal anti-RXRα	Proteintech	Cat #:21218; RRID:AB_10693633
Rabbit polyclonal anti-HSC70	Proteintech	Cat #:10654; RRID:AB_2120153
Rabbit monoclonal anti-LAMP2A	Abcam	Cat#:ab125068; RRID:AB_10971511
Rabbit monoclonal anti-β-actin	Cell Signaling	Cat #:4970; RRID:AB_2223172
Rabbit polyclonal anti-α-actinin	Proteintech	Cat #:11313; RRID:AB_2223815
Rabbit polyclonal anti-GAPDH	Sigma	Cat #:67165; RRID:AB_796208
Rabbit polyclonal anti-ALDH1A2	Proteintech	Cat #: 13951; RRID:AB_2224033
Rabbit polyclonal anti-ALDH1A3	Proteintech	Cat #: 25167; RRID:AB_2879937
Bacterial and virus strains
Sh-humanALDH1A1(5′ to 3’):GAACAGUGUGG GUGAAUUGTT	GENEWIZ	N/A
Biological samples
Human bladder cancer tissues and adjacent tissues	The affiliated hospital of Qingdao university	N/A
Chemicals, peptides, and recombinant proteins
DMSO	Sigma-Aldrich	Cat #: D2650
Nicotinamide	MCE	Cat #:HY-B0150
MG132	MCE	Cat #:HY-13259
TSA	MCE	Cat #:HY-15144
Gemcitabine	MCE	Cat #:HY-17026
cisplatin	MCE	Cat #:HY-17394
NCT501	MCE	Cat #:HY-18768
ATRA	MCE	Cat #:HY-14649
RALD	MCE	Cat #:HY-W004500
CQ	MCE	Cat #:HY-17589A
CHX	MCE	Cat #:HY-12320
CIKEAAGK(2-Hydroxyisobutyric acid)SNLKR-NH2	QYAOBIO	Cat #:4209-M
CIKEAAGKSNLKR-NH2	QYAOBIO	Cat #:4209-C
Critical commercial assays
MTT reagent	R&D Systems	4890-25-01
Annexin V-FITC/PI detection kit	Yeasen	40302ES50
Acetaldehyde Dehydrogenase(ALDH) Activity Assay Kit	Solarbio	BC0755
HiScript II One Step qRT-PCR SYBR Green Kit	Vazyme	Q221
Deposited data
RNA-seq data	TCGA-BLCA	https://portal.gdc.cancer.gov/repository
Experimental models: Cell lines
T24	the Chinese Academy of Sciences Cell Bank	SCSP-536
UMUC3	the Chinese Academy of Sciences Cell Bank	TCHu217
HEK293T	the Chinese Academy of Sciences Cell Bank	GNHu17
Experimental models: Organisms/strains
BALB/c nude	Vital River Laboratories, Beijing, China	N/A
Oligonucleotides
Si-humanALDH1A1(5′ to 3’):GAACAGUGUGGGU GAAUUGTT	Genepharma	N/A
Si-humanHDAC2(5′ to 3’):UCCGUAAUGUUGCU CGAUGTT	Genepharma	N/A
Si-humanHDAC31(5′ to 3’):AAUAUCCCUCUACUC GUGCUGA	Genepharma	N/A
Si-humanLAMP2A(5′ to 3’):CUGCAAUCUGAUUGA UUAUUTT	Genepharma	N/A
qPCR Primer sequences please see Table S1	This paper	N/A
Recombinant DNA
cDNA-Human ALDH1A1	GENEWIZ	N/A
cDNA-Human ALDH1A1K260T	GENEWIZ	N/A
cDNA-Human ALDH1A1K260R	GENEWIZ	N/A
cDNA-Human HDAC2	GENEWIZ	N/A
cDNA-Human HDAC3	GENEWIZ	N/A
Software and algorithms
SPSS statistics 22.0	IBM	https://www.ibm.com/spss
GraphPad Prism 8.0	GraphPad	https://www.graphpad.com/scientifificsoftware/prism/
R version 4.1.0	The R Foundation	https://www.r-project.org
NovoExpress 1.6.1	Agilent	https://www.agilent.com.cn/
ImageJ	Schneider et al.84	https://imagej.nih.gov/ij/