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. Author manuscript; available in PMC: 2024 Oct 1.
Published in final edited form as: J Hepatol. 2023 Jun 20;79(4):1025–1036. doi: 10.1016/j.jhep.2023.05.045

Fig. 5. Long-term depletion of neutrophils in chronic liver injury leads to a decrease in proliferating biliary epithelial cells.

Fig. 5.

(A) Immunohstochemistry analysis of livers of control- and DDC-treated mice with anti-Ly6G antibody (1A8) or isotype (n = 4 mice per group). Scale bars: 100 μm. (B) Staining of progenitor markers in mice treated with anti-Ly6G antibody (1A8) and isotype. Scale bars: 100 μm. (C) Immunofluorescence of KRT19 and CCND1 of liver sections of mice treated with anti-Ly6G antibody (1A8) and isotype (n = 3 mice per group). Scale bars: 50 μm. All data is presented as mean ± SEM. *p <0.05, **p <0.01 and ***p <0.001 as determined by one-way ANOVA with Tukey’s multiple comparison test (MPO, KRT19, S/R in A), one-way ANOVA Kruskal-Wallis with Dunn’s multiple comparison test (CD31 in A) and Mann-Whitney test (C). CCND1, cyclin D1; CD31, cluster of differentiation 31; DDC, 3,5-diethoxycarbonyl-1,4-dihydrocollidine; EpCAM, epithelial cell adhesion molecule; ISO, isotype; KRT, cytokeratin; MPO, myeloperoxidase; Ly6G, lymphocyte antigen 6 complex locus G6D; SOX9, SRY-Box transcription factor 9; S/R, sirius red.