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. 2023 Oct 16;10(10):ENEURO.0054-23.2023. doi: 10.1523/ENEURO.0054-23.2023

Figure 1.

Figure 1.

Characterization of tdTomato (tdT) reporter expression driven by the ΔNp73-Cre or the Wnt3a-Cre mouse line. A, B, Comparison of tdT reporter expression in the ΔNp73-Cre; LSL-tdTomato mice at P18 (A) and the Wnt3a-Cre; LSL-tdTomato mice at P20 (B). Left, Representative images show tdTomato expression in the hippocampus. In the hippocampus of both mouse lines, reporter expression is limited to the DG and along the hippocampal fissure (HF). Reporter expression was not detected in cornu ammonis (CA) regions. Scale bars, 200 μm. Middle, Higher-magnification images show reporter expression in the DG. In both transgenic lines, reporter expression is readily detectable in Cajal–Retzius cells (white arrows) located along the HF and in the dentate gyrus molecular layer (ML). While cells in the granular layer (GL; yellow arrows) of the ΔNp73-Cre; LSL-tdTomato mice are only occasionally observed to express tdTomato, there is extensive tdTomato expression in the granular layer in the Wnt3a-Cre; LSL-tdTomato mice. Scale bars, 200 μm. Right panels, Cajal–Retzius cells are identified by their unique tadpole-like morphology and the expression of RELN. Scale bars, 10 μm. SLM, Stratum lacunosum-molecular; V3, third ventricle.