Figure 10.

Effects of neonatal intraventricular adeno-associated virus injection on ΔNp73-lineage cells in the third ventricle and choroid plexus. A, Wild-type or ΔNp73-Cre pups injected at P0 with AAV8/EF1a-DIO-ChR2-mCherry at different titers were analyzed for mCherry expression at P14. At 1.0 × 10¹³ viral titer, Cre-independent recombination was evident in the wild-type mice (white arrowheads). Additionally, at 1.0 × 10¹³ GC/ml viral titer, choroid plexus epithelial cells in the third ventricle (3V) of the ΔNp73-Cre mice were also transduced, evident from their mCherry expression (yellow arrows). Transduction of epithelial cells is not detectable at the 1.0 × 10¹² AAV titer. Scale bars, 300 μm. B, Wild-type or ΔNp73-Cre pups injected at P0 with AAV8/EF1a-DIO-ChR2-mCherry or AAV8/hSyn-DIO-hM3D-mCherry at 1.0 × 10¹² GC/ml viral titer were analyzed for mCherry expression at 7 weeks postinjection. Reporter expression is absent from ependymal and choroid plexus cells.