Figure 2.

Reporter expression in the ΔNp73-Cre; LSL-tdTomato mice. A, Left, Representative confocal images show tdTomato (tdT) reporter expression in the ΔNp73-Cre; LSL-tdTomato mice at P7, P17, 4 weeks (4W), and 7W. Right panels, High-magnification images are shown with coimmunostaining for tdTomato and the Cajal–Retzius (CR) cell marker TRP73. TRP73⁺ CR cells expressing tdTomato are marked with white arrows, while TRP73^– granule neurons of the dentate gyrus are marked with yellow arrows. SLM, Stratum lacunosum-molecular; HF, hippocampal fissure; GL, granular layer; ML, molecular layer. Scale bars: whole dentate gyrus, 200 μm; higher magnification, 20 μm. B. Left, An image of a Nissl-stained mouse brain coronal section depicts the areas of interest. loML, Lower ML (i.e., ML of the infrapyramidal blade). Right, Quantification of tdTomato⁺ CR cells and granule neurons, including quantification of CR cell-targeting specificity. Data are presented as scatter plots with all data points shown and error bars representing ±SD. Each data point is an individual animal, whereby three sections were measured for each animal. Statistical analyses were performed using nested one-way ANOVA with Tukey’s post hoc test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. The data that illustrate reporter expression in the neocortex are shown in Extended Data Figure 2-1. The images that demonstrate granule neuron identity are shown in Extended Data Figure 2-2. Analyses of the effect of sex on tdTomato-expressing granule neurons are provided in Extended Data Figure 2-3.