Figure 9.
Effects of neonatal intraventricular adeno-associated virus injection on ΔNp73-lineage cells in the neocortex. ΔNp73-Cre pups injected at P0 with AAV8/EF1a-DIO-ChR2-mCherry were analyzed for mCherry expression at 14 d (P14, top) or 7 weeks (bottom) post injection. Coimmunostaining of TRP73 and RELN identifies neocortical Cajal–Retzius cells that express mCherry (yellow arrows). Neocortical Cajal–Retzius cells are only found in layer 1 (L1). They are very sparse at P14 and almost completely disappear by 7 weeks of age. Scale bars, 30 μm.
