Table 1.
List of genotyping PCR primers used in this study
| Gene/allele target | Primers (5′−3′) | PCR product size |
|---|---|---|
| Cre (generic) | CCGGGCTGCCACGACCAA | 490 bp |
| GGCGCGGCAACACCATTTTT | ||
| LSL-tdTomato | AAG GGA GCT GCA GTG GAG TA | Mutant = 196 bp |
| CCG AAA ATC TGT GGG AAG TC | WT = 297 bp | |
| GGC ATT AAA GCA GCG TAT CC | ||
| CTG TTC CTG TAC GGC ATG G | ||
| LSL-HA-hM3D | CGCCACCATGTACCCATAC | Transgene/mutant = 204 bp |
| GTGGTACCGTCTGGAGAGGA | Internal positive control/WT = 297 bp | |
| AAGGGAGCTGCAGTGGAGTA | ||
| CCGAAAATCTGTGGGAAGTC | ||
| LSL-ArchT | AAG GGA GCT GCA GTG GAG TA | Mutant = ∼300 bp |
| CCG AAA ATC TGT GGG AAG TC | WT = 297 bp | |
| ATT GCA TCG CAT TGT CTG AG | ||
| CCG AAA ATC TGT GGG AAG TC | ||
| LSL-ChETA | CACTTGCTCTCCCAAAGTCG | Mutant = ∼300 bp |
| TACTCCGAGGCGGATCACAAGC | WT = 452 bp | |
| ACTAGTCAATAATCAATGTCGACCGG | ||
| ΔNP73-Cre specific | ATTCTCCCACCGTCAGTACG | WT = 328 bp |
| GAATGCCAACTCTCAGTCCG | KO = 796 bp | |
| GTCTCTCTGAACCCCAACCA |