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. 2023 Sep 8;10(10):uhad177. doi: 10.1093/hr/uhad177

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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MaMADS1 directly binds to the MaNAC083 promoter. (A) Y1H study of MaMADS1's interaction with the MaNAC083 promoter. (Left) The MaNAC083 promoter showed no basal expression in yeast grown on Leu-deficient SD media supplemented with 500 ng mL⁻¹ AbA. (Right) After transforming plasmids containing cassettes that constitutively express the MaMADS1 effector or negative control (pGADT7), a yeast growth assay was performed on Y1H reporter strains. The interaction was evaluated by yeast growth after transformation on SD media without Leu in AbA's presence. (B) MaMADS1 binding to MaNAC083 promoter in an EMSA assay. The probe sequences for ethylene biosynthetic gene promoters are displayed at the top. DNA–protein complexes from probes exposed to pure GST (negative control) or recombinant GST-MaMADS1 protein were resolved on native polyacrylamide gels. Triangles show competing quantities of unlabeled wild-type or mutated probes.