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. 2023 Oct 19;42:270. doi: 10.1186/s13046-023-02820-z

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Effects of UGDH knockdown in C1/MES cell lines, and over-expression in C4/DIF cell lines on adherent and spheroid growth in vitro. Western blot and densitometry analysis of UGDH expression in C1/MES cell lines (A) OV90 cells, and (B) HEYA8 cells, with DOX-inducible negative control shRNA (shneg) or DOX-inducible shRNA targeting UGDH (sh459, sh939) after 3 days of DOX induction in adherent (Adh.) or spheroid (Sph.) culture conditions. Western blot and densitometry analysis of UGDH expression in C4/DIF cell lines: C ACI23 cells, and (D) SKOV3 cells with stably expressed vector control (VC) or UGDH (Ov, OverX) grown for 3 days in, and adherent (Adh.) or spheroid (Sph.) culture conditions. Viability of C1/MES cells grown in adherent or spheroid conditions with control (shneg) and UGDH-knockdown (sh459,sh939): E OV90, F HEYA8. Viability of C4/DIF cells grown in adherent or spheroid conditions with control (VC) and UGDH overexpression (OverX): G ACI23, H SKOV3. Sphere forming capacity of C1/MES cell lines with control (shneg) or UGDH knockdown (sh459, sh939) in (I) OV90 and (J) HEYA8. Sphere forming capacity of C4/DIF cell lines with control (VC) or UGDH overexpression (OverX): K ACI23, L SKOV3. n.s non-significant, *p < 0.05, **p < 0.01, ***p < 0.001