. Author manuscript; available in PMC: 2023 Oct 19.

Published in final edited form as: Mol Cell Endocrinol. 2022 Jul 14;554:111724. doi: 10.1016/j.mce.2022.111724

Table 1. List of selected differentially expressed osteoclast specific genes from RNA-sequencing experiments comparing low [Na⁺] versus normal [Na⁺] effects.

Samples were from RAW264.7 osteoclastic cells exposed for 24h to media with low [Na⁺] (120 mmol/l) or normal [Na⁺] (140 mmol/l). P < 0.05 was considered statistically significant.

Gene name	Protein	Protein function	Fold change	p-value	FDR q-value	FPKM of gene in samples		FPKM of gene in samples

						Mean low [Na+] mean	S.D. low [Na+]	Mean normal [Na+]	S.D. normal [Na+]

	Top selectively up regulated genes
	osteoclast specific functions
Acp5	tartrate resistent acid phosphatase	matrix degradation	119	1.14E-09	1.42072172426566E-05	956.869	20.332	7.048	0.107
Ctsk	cathepsin K	matrix degradation	41	8.06E-09	3.23715251716872E-05	1091.835	23.539	25.497	0.615
Car2	carbonic anhydrase 2	pH regulation	15.1	1.95E-08	3.23715251716872E-05	191.299	2.109	11.110	0.335
Ank	ankyrin	Na ion transport, motility	14.7	3.3321053150015E-07	9.37992336989715E-05	549.470	31.474	36.470	0.330
Mmp9	Matrix metalloproteinase 9	matrix degradation	5.3	1.05E-06	0.000125719239559815	226.789	10.066	41.638	0.806
Src	src kinase	polarization and attachment	2.7	0.0114380736211345	0.0236459993943777	48.751	0.531	23.527	6.642
Atp6v0d2	vacuolar ATPase	acid secretion	1.8	7.84839173536556E-05	0.000861393723299428	276.710	11.962	155.120	3.812
	osteoclast regulators
Dmpk	effector of Rho GTPase	osteoclast formation	13.7	1.61876136162142E-07	6.99679395159155E-05	18.940	0.926	0.457	0.010
ocstamp	osteoclast stimulatory transmembrane protein	osteoclast differentiation	13.1	1.12945768959705E-07	6.5264752779995E-05	16.122	0.211	0.307	0.054
Gsttl	glutathione-S-transferase theta 1	osteoclast formation	11.7	1.12896815884778E-05	0.000333377019195173	23.545	1.471	1.111	0.237
Traf1	receptor associated factor 1	osteoclast formation	5.3	2.89036713230217E-07	9.37992336989715E-05	14.308	0.357	1.877	0.074
Slc9b2	Na+/H+ antiporter	osteoclast differentiation	11.2	2.6482004722439E-07	8.94733894689757E-05	10.568	0.525	0.026	0.020
Oscar	osteoclast associated receptor	osteoclast differntiation	9.6	8.70E-07	0.000119460779583085	9.522	0.504	1.033	0.102
Myc	c-Myc proto-Oncogen	osteoclast differntiation	8.2	3.89079166107642E-07	9.40352645726255E-05	55.079	1.686	5.872	0.253
Fos	c-Fos proto-Oncogen	osteoclast proliferation and survival	6.8	1.08096280249104E-06	0.000127482226357929	82.766	2.079	11.556	0.609
Nfatc1	nuclear factor of activated T cells	osteoclast differntiation	5.9	1.14730317208878E-05	0.000333377019195173	48.814	4.073	7.336	0.397
dcstamp	dendritic cell-specific transmembrane protein	osteoclast differntiation	2.4	0.019	0.03525231098499	18.052	1.065	7.483	7.004
Siglec15	sialic acid binding lectin	osteoclast differntiation	2	0.018	0.0334524480315347	5.618	1.392	2.247	0.478
cdc26	cell division cycle 26	osteoclast formation	1.9	0.000542406918978777	0.00266953893470618	5.234	0.522	2.293	0.097
Sirt3	sirtuin 3	osteoclast activity	1.6	6.91034552151581E-05	0.00081113830389173	2.212	0.063	1.050	0.065
	Top selectively down regulated genes
Hpse	heparanase	inhibits osteoclastogenesis	−4.9	4.03165376905434E-06	0.000201638691036798	1.341	0.331	66.318	1.006
Cd200r4	CD200 receptor	inhibits osteoclast differentiation	−4.6	1.37942238239708E-05	0.000357775809762266	2.428	0.530	79.541	5.138
Tspan10	tetraspanin10	inhibits osteoclastogenesis	−3.9	2.63749230677846E-07	8.94733894689757E-05	2.270	0.069	49.365	2.571
Atf3	activating transcription factor 3	inhibitor of osteoclast proliferation	−3.6	3.84626621108275E-06	0.000201638691036798	10.542	0.765	137.453	9.880
Gstm1	Glutathione S-Transferase Mu 1	inhibits focal adhesion	−3.4	6.36292020739226E-06	0.00025742027673984	19.035	0.351	214.016	23.478

Table 1. List of selected differentially expressed osteoclast specific genes from RNA-sequencing experiments comparing low [Na+] versus normal [Na+] effects.

Table 1. List of selected differentially expressed osteoclast specific genes from RNA-sequencing experiments comparing low [Na⁺] versus normal [Na⁺] effects.