Table 4.
List of significantly affected pathways by low [Na+] based on data from whole genome microarray analysis.
| PROCESS | Low [Na+]/Normal [Na+] | |
|---|---|---|
|
|
||
| % enrichment | −log p value | |
|
| ||
| Sodium ion transport | 100 | 129 |
| Cell cycle regulation | 68 | 50 |
| Cell-matrix interactions | 10.4 | 38 |
| Osteoclast differentiation | 16.8 | 31 |
| Inhibition of Ca++ transport via voltage-gated calcium channel | 28 | 31 |
| NFκB pathway | 39 | 27 |
| Cell motility | 29.8 | 24 |
| Negative regulation of spermatogenesis | 42 | 16 |
| Skeletal development | 29.3 | 14 |
| ECM proteolysis | 24 | 13 |
| Positive regulation of cell proliferation | 14.5 | 10 |
| TGF-β Signaling | 73 | 8 |
| p53 signaling | 68 | 8 |
| Oxidative stress | 70 | 7 |
| Cell adhesion | 30.8 | 7 |
| Ossification | 10 | 6 |
| Osteoporosis | 81 | 5 |
| Angiogenesis | 10 | 4 |
| L-ascorbic acid transport | 25 | 4 |
Results confirmed RNA-sequencing findings on top physiological processes selectively modified by for 24h exposure to low [Na+] (120 mmol/l) compared to normal [Na+] (140 mmol/l) in RAW264.7 murine osteoclastic cells. The −log p value above 1.5 is considered statistically significant. Processes are listed by −log p-value ranking.